The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence intensity, glow luminescence, fluorescence polarization and time-resolved fluorescence (TRF). When a feature or capability applies to only certain readers, this exception is noted. Plate chamber design of the SpectraMax category 1 microplate readers. Figure 1. Add to Cart. 72 MB. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Sale. . 40266 Email: drugdiscoverytech@lifetech. 5. 306 plate air cal fail,low light. I have been using the SpectraMax M5 since 2011. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. 9000-0756. SpectraMax Injector Cartridge with SmartInject™ Technology. spr SpectraMax Plus 384. . Fluorescence Microplate Reader Comparison Chart. 1. VWR offers biophotometers for your lab's needs. . edu) to be added as a trained user in iLab. Click SoftMax Pro Software User Guide. Overall, SpectraMax M5 is a powerful lab tool that combines advanced detection capabilities, user-friendly software, and comprehensive accessory options. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . Maximum signal with lowest background. The assay window spanned four orders of magnitude. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 4. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Place the plate into a SpectraMax M5 e plate reader (Molecular Devices). Microplate Reader Shelf. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. e. General procedure forpreparedviscosity analysis The parent stock solution was prepared by dissolving probe FCV (5 mM) in DMSO. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). . SoftMax® Pro Microplate Data Acquisition and Analysis Software serves as the user interface to the microplate reader. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. The assay applies fluorescence polarization to quantify Fc-containing IgG. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M5 Multi-Mode Microplate Reader. . The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Offer Ends In: 18 Days 19: 38: 21. ORDER. The M5 can read volumes down to 2uL with the. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. 315 can't find zero order. The SpectraMax ABS Plus reader utilizes Molecular . The Spectr. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. The following document is intended to demonstrate setup of this. The guide also covers the software features, the specifications, and the accessories of the systems. SpectraMax DuoLuc Reporter Assay (SMP 6. The M5 has two PMTs. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. . Microplate Readers include the M3, M4,. e. you can use a resource. 1. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Principle of the HTRF IP-One competitive binding assay. 1. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. spr SpectraMax iD3 SpectraTest ABS2 v1. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). D 1. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5 Multi-Mode Microplate Reader. spr SpectraMax iD5 SpectraTest ABS2 v1. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. ISM Equipment Classification (Group 1, Class A) This equipment is. 3: The cuvette chamber. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Step 4. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Description. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. . Call 516-939-0888 to check current. Lift up the lid over the chamber to insert or remove a cuvette. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. 1. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader Why does "Path?" appears in all wells when PathCheck is selected? Cause: Water Constants. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 72 MB. Choose from a three- (M3), four- (M4), or Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Select the fluorescence option and read the total fluorescence. 1. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Page: 1/6. Add to Cart. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images were obtained with SU8010 field. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. 190-1000. HTRF is a proprietary time-resolved fluorescence technology that overcomes The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. 4. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. Choose from a three- (M3), four- (M4), orSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 3: The cuvette chamber. Absorbance (UV – VIS) Fluorescence; Glow luminescence; Time-resolved fluorescence; Fluorescent polarization; ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated. , ~488 nm), has the optical. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 5. . deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. +44-118-944-8000. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Settings not required by a reader are indicated by ‘---’. . Please Inquire. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Table of Contents. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataSpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. free shipping. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. HEALTH AND SAFETY CONSIDERAIONS The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 313 reference gain check fail. Wavelength Range. 3. TR-FRET assay. 7°C (gradient heating design). Spectral Range. Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The excitation and the emission wavelengths on. We would like to show you a description here but the site won’t allow us. SpectraMax M3, M4, and M5/M5e readers. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Select the fluorescence option and read the total fluorescence. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. When the user applies a set temperature of 37°C, the top is heated to 37. Proceed with the following steps to optimize the instrument: 1. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. Wavelength Range. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Description 1. 315 can't find zero order. 00 USD. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Molecular Device SpectraMax M5 Plate Reader - Certified with Warranty. 4. Sensitive fluorescent quantitation of DNA with the Quant-iT. Examples: “Press carefully to engage,” “ Do not. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. ATP standard curves read on SpectraMax microplate readers. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. • Tel. ® reader to the computer. . Published Dec 16, 2020 | Updated Dec 16, 2020. 0 out of 5 $ 15,999. 190-1000 nm, tunable in 1 nm increments. . The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Molecular Devices SpectraMax M5 Multimode Microplate Reader. 2. Supplier: Molecular Devices. Wash Buffer Concentrate was diluted 10-fold. Also refer to this Multimode Reader Comparison Chart. Features. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . In the M2, M5 and Plus. 6. The ability to measure inhibition of phosphorylation by Erlotinib was tested by incubating cells with increasing concentrations of Erlotinib, then stimulating cells with 0. 1. 2. Monday to Friday 7am - 5pm Pacific Time. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9THE SPECTRAMAX PLUS Two ways to correct pathlength The SPECTRAmax PLUS gives you two options when making pathlength-cor-rected measurements: 1) putting the aqueous solvent into a clean 1 cm reference cuvette in the instrument’s cuvette port and having the instrument base the calcu-lations on the A 1000 and A 900Molecular Devices社の製品: マイクロプレート リーダー用ソフト SoftMax® Proに関するすべての情報をご覧ください。価格、見積もり、お近くの販売店を知るにはメーカーまたは本社に直接お問い合わせくだ. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The dynamic range of the assay was approximately 5 orders of magnitude. Read fluorescence polarization at room temperature (RT, 19–25°C). SpectraMax Spectrofluorometers. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 1. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. ) Open SoftMax Pro, set Preference to SpectraMax L, and try the different available COM ports to find the one for the SpectraMax L. . The M5 can read volumes down to 2uL with the uMax Low Volume Plate. Download PDF versions of the SpectraMax M5 User Guides from this page. SpectraMax M5, Analyst HT or Analyst GT. Step 1: Open the SoftMax Pro IMAP FP protocol. 2. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax Glo and DuoLuc. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. 6: bioNova científica, s. Step 2. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Spectramax M5 plate reader (96/384 format) Multimode photometrics for endpoint or kinetic measurements. No filter change with dual monochromator optics. With optimized reagents, option to operate the. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit The Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. The dual monochromator optics allow the widest range of applications to be utilized for bior. edu) to be added as a trained user in iLab. UV/Vis Photometric Performance:. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. After 4 h, the fluorescence intensity of serum FITC-dextran was obtained at 480 nm excitation wavelength and 520 nm emission wavelength using a Spectramax M5 microplate reader (Molecular Devices). . The SpectraMax M5 is a microplate reader that can be used for various applications,. Category 2 and 3 microplate readers use gradient heating as well. Detection modalities are shown in Table 1-1. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 1) SpectraMax Glo Steady Luc Reporter Assay (6. D 1 1. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. Download PDF versions of the SpectraMax M5 User Guides from this page. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. . User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. NORTH AMERICA. 2. ScanLater Western Blot Detection Cartridge. Bookings are. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. MDS Analytical Technologies announced the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers. 7°C (gradient heating design). . Operated fromFluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. acquired on a SpectraMax M5 (Molecular Devices). For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. M5. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. ” SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. 7°C and the bottom to 36. Category 2 and 3 microplate readers use gradient heating as well. 40266 Email: drugdiscoverytech@lifetech. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Choose from a three- (M3), four- (M4), or SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity,. 190-1000. High tolerance for contaminants. . The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. ItSpectraMax® M5多功能读板机为使用者提供了除化学发光以外的其他检测方法，例如：光吸收，荧光强度，荧光偏振和时间分辨荧光。 SpectraMax® L和SpectraMax® M5酶标仪均用SoftMax® Pro软件进行操控和设置，该软件含有CellTiter-Glo检测模板和其他120多个分析模板，这些. 7 nM EGF, the EC 80 concentration determined from. 2 mm long, 86. Table of Contents. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. Step 1. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Set the excitation at 531 nm and emission at 595 nm. . . 5. D 1. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 7. Last lab had ours for years and never had a problem with it. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Optimal instrument settings are the same for all three readers. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. The substrate solution (100 μL/well) was subsequently employed for 5-min reaction and the absorbance at 370 nm was monitored on a SpectraMax M5 plate reader (Molecular Devices). Open the Softmax Pro software and select the FP option. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance (ANOVA) (Table 1). 0 (IBM Corporation, Armonk, NY, USA). Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. Offer Ends In: 18 Days 19: 38: 21. The SpectraMax M5 Multi-Mode. SpectraMax L Microplate Reader provides medium to high throughput. com A. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. NFkB Translocation is Proteasome-Dependent The proteasome is a large protein complex that degrades proteins into short peptides in a ubiquitin-dependent manner. For example, microplates containing different samples can be read using the312 gain calibration failed. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. For example, microplates containing different samples can be read using the same orOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). Cell-based experiments In the first experiment, we prepared a dilution series of CHO-K1 cells transiently transfected withThe SpectraMax 190 reader is the tool for DNA analysis. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. For more information, please visit us at Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Open the SoftwarePro® Data Acquisition & Analysis software,. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Microplate Reader Shelf. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. l. Table of Contents. Molecular Devices SpectraMax PLUS 384 Spectrophotometer System-New ABs1 Performance Testing August 2021. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. 3. , ~488 nm), has the optical. Step 2. Published Dec 16, 2020 | Updated Dec 16, 2020. DOCUMENTATION. Maximizes fluorescent signal from a wide range of cell types by reducing cell wash artifacts. 多功能酶标仪SpectraMax M5e. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. . 1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 3. 3. ) Turn on the SpectraMax L and connect the computer to the SpectraMax L using the USB cable. 2. We would like to show you a description here but the site won’t allow us. The Spectramax performs spectral scanning in 1 nm increments, and up to six wavelengths. Note: In this user guide, all references to SpectraMax Multi-Mode. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. If you do not have an iLab account you must first make one: SpectraMax Paradigm Fluo rescence Polarization (FP) Detection Cartridge is a single slot detection cartridge and occupies one (1) slot. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). For example, microplates containing different samples can be read using the The interval between reads was set at 15 s or 60 s, respectively. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. The time course of the cleavage reaction was monitored by loss of FRET (excitation, 450 nm; emission, 655 nm) with 15 reads/well and 4–5-s read intervals on a SpectraMax M5 plate reader (Molecular Devices). Fix: While proper use of the Shake button is to hold the button down for the duration of the desired mixing time, repeated sequential clicking is now allowed. . . The chamber. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Customer Satisfaction is our number one goal All lab equipment in stock. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Recertification is recommended every two years. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. 1. 190-1000 nm, tunable in 1 nm increments. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Bookings are free, but you will need to use iLab to book. Description. , contact Molecular. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology.